Direct Immunofluorescence (DIF)
Specialized testing for autoimmune and inflammatory skin diseases using direct immunofluorescence techniques.
Expert DIF Testing
Direct Immunofluorescence (DIF) is a specialized technique used to detect immune deposits in tissue samples. This testing is essential for diagnosing autoimmune blistering diseases, connective tissue disorders, and other inflammatory skin conditions.
Specialized Handling
Proper transport and processing of fresh frozen specimens
Expert Interpretation
Board-certified dermatopathologists with DIF expertise
Comprehensive Panels
Complete antibody panels for accurate diagnosis
Conditions Diagnosed
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Pemphigus vulgaris/foliaceus
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Bullous pemphigoid
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Linear IgA disease
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Dermatitis herpetiformis
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Lupus erythematosus
DIF Antibody Panels
We offer comprehensive antibody panels tailored to specific clinical presentations and suspected diagnoses.
Standard DIF Panel
- IgG
- IgA
- IgM
- C3
- Fibrin
Comprehensive screening for most autoimmune conditions
Extended DIF Panel
- Standard panel plus:
- IgG4
- C1q
- Albumin
- Kappa/Lambda light chains
Enhanced testing for complex cases
Specimen Requirements
Fresh Frozen Tissue
- • 4mm punch biopsy or larger
- • Perilesional normal-appearing skin
- • Transport in saline-moistened gauze
- • Ship on wet ice (not dry ice)
Transport Guidelines
- • Ship within 24 hours of biopsy
- • Use overnight shipping
- • Include completed requisition
- • Avoid freezing or formalin
Download Requisition Forms
Download the required requisition forms for submitting DIF specimens. Proper documentation is essential for accurate testing.
DIF Standard Requisition
Standard form for direct immunofluorescence testing with clinical history and specimen details.
Transport Instructions
Detailed guidelines for proper specimen collection, handling, and transport for DIF testing.
Questions About Specimen Collection?
Our technical team can provide guidance on proper specimen collection and transport for DIF testing.
Ready to Get Started?
Contact us today to learn more about our DIF testing services or to discuss specimen requirements.